In vitro fertilization (IVF) is a technique in which oocytes are fertilized by sperm in the laboratory. After recovery of the follicular puncture the successful embryos are observed a few days, until the moment that the best quality embryos are transferred to the uterus of the mother.

The insemination of the oocytes can be performed with the sperm of the couple (homologous IVF) or with sperm from a donor (heterologous IVF).

The requirements for a couple to undergo in vitro fertilization and to obtain the greatest chance of success are:

  • The sperm sample, has to have a suitable motility and count parameters, otherwise we will have to combine an artificial insemination treatment.
  • Exclude possible malformations of the uterus through an ultrasound that could affect fertilization, implantation and pregnancy. Furthermore, we need to ensure a good response to the ovarian stimulation, in order to obtain sufficient oocytes for the performance of the technique.


  • Female sterility:

- Ovulation problems

- Blocked fallopian tubes or the absence of the fallopian tubes

- Moderate or severe endometriosis that cause infertility

- Age of women

  • Male sterility:

- Mild moderate changes in the parameters of sperm count and / or moderately decreased motility of the sperm

- Failure of previous artificial insemination

- Unexplained infertility

  • IVF with donor sperm is recommended to:- Women who are facing motherhood alone- Female homosexual couples
  • Severe male factor:- Azoospermia: no sperm in the ejaculate- Genetic diseases which can not be diagnosed by PGD- Sexually transmitted diseases


IVF treatment involves the following phases:

Ovarian stimulation
We begin treatment with ovarian stimulation, where we use a number of hormones similar to those produced by the woman herself (gonadotropins). The goal is the development of multiple follicles to abtain a high numer of oocytes and thus increase the chances of success. The stimulation is regularly monitored by ultrasound and blood tests.
Ovulation induction

After several follicles have reached the right size (18 mm diameter) HCG hormone is administered to trigger final oocyte maturation, which will occur after 34-36 hours and that is when will be performed the follicular puncture.

Follicular puncture
Under sedation, the fertility specialist removes mature oocytes by follicular puncture and vaginally. The oocyte retrieval is a minimally invasive procedure that normally takes less than 15 minutes. Generally, patients can resume normal activities the next day.
Sperm capacitation
On the day of follicular puncture, the andrology laboratory has prepared the semen sample (seminal training) by previously selecting sperm washing techniques with greater motility.
When using a semen from a sperm bank we will proceed to the thawing of the sample and the training.
Insemination of oocytes
Depending on the alterations or chromosomal genetics to be analyzed and / or semen quality we will proceed with the insemination of oocytes by conventional IVF or ICSI.
Fertilization and embryo culture
The day after insemination, fertilization is observed in the oocytes. Those who have been properly fertilized will be kept under observation in the laboratory for several days (between 2 and 5 days). Day after day we will be watching the embryos dividing and increasing its number of cells.
Embryo transfer
Embryos that have, after genetic analysis, normal genetic and chromosome endowment and / or adequate morphological characteristics will be trasferred to the womb, and if there are healthy embryos leftover we will proceed to freeze. The day of the transfer we will decide the appropriate number of embryos to be transferred, those can not be higher than three per cycle. The gynecologist uses a small catheter to guide the embryos through the cervix and deposit them. The embryo transfer procedure lasts a few minutes and the recovery time for the patient is minimal.
Pregnancy test

The pregnancy test will be done trough a blood test 13 days after the follicular puncture.

If the patient has lost blood before undergoing the test it is essential to never leave the medication and to contact the medical team for advice. Throughout treatment, your personal assistant will be available at all times to resolverle doubts that you may have and support you in this special moment.

Fertility clinics in Spain

Medical Team

Susana Sempere - Gynaecology

Dr. Susana Sempere Ferri

Antonio Moya - Reproductive Medicine

Dr. Antonio Moya

Eva Moreno

Dr. Eva María Moreno Ruiz

Jose Manuel Lozano - Gynaecology

Dr. Jose Manuel Lozano

Romina Ramírez - Gynaecology

Dr. Romina Ramírez Melguizo

You know what…

  • In general, the pregnancy rate hovers around 30-40% per cycle in women under 38 years. Above the age of 40, the figures are significantly lower (10-15%). Anyway, we should note that these are general rates from couples that suffer from infertility.
  • The first test tube baby, Loise Brown, was born on June 25, 1978 in the UK. In Spain it was July 12, 1984.
  • The IVF treatment is not painful, but in some phases of the cycle it can produce anxiety, especially after the embryo transfer, when you wait for the result.
  • Usually after the transfer, the patient waits about an hour in the room of the fertility clinic. If your gynecologist does not indicates so, rest is not needed after the transfer. There are no studies showing that resting increases the chances of pregnancy. Then, once home, they can carry out their normal life.

More Information about In Vitro Fertilization in Spain